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J Vet Sci. 2018 Jan 31;19(1):89-98. doi: 10.4142/jvs.2018.19.1.89.

A new recombined porcine reproductive and respiratory syndrome virus virulent strain in China.

Author information

1
Animal Science College & National Engineering Center for Swine Breeding Industry, South China Agriculture University, Guangzhou 510642, China.
2
School of Animal Husbandry and Medical Engineering, Xinyang Agriculture and Forestry University, Xinyang 464000, China.

Abstract

Porcine reproductive and respiratory syndrome (PRRS) is one of the most important swine diseases worldwide. In the present study, a new virulent strain of PRRS virus (PRRSV), GDsg, was isolated in Guangdong province, China, and caused high fever, high morbidity, and high mortality in sows and piglets. The genome of this new strain was 15,413 nucleotides (nt) long, and comparative analysis revealed that GDsg shared 82.4% to 94% identity with type 2 PRRSV strains, but only 61.5% identity with type 1 PRRSV Lelystad virus strain. Phylogenetic analysis indicated that type 2 PRRSV isolates include five subgenotypes (I, II, III, IV, and V), which are represented by NADC30, VR-2332, GM2, CH-1a, and HuN4, respectively. Moreover, GDsg belongs to a newly emerging type 2 PRRSV subgenotype III. More interestingly, the newly isolated GDsg strain has multiple discontinuous nt deletions, 131 (19 + 18 + 94) at position 1404-1540 and a 107 nt insertion in the NSP2 region. Most importantly, the GDsg strain was identified as a virus recombined between low pathogenic field strain QYYZ and vaccine strain JXA1-P80. In conclusion, a new independent subgenotype and recombinant PRRSV strain has emerged in China and could be a new threat to the swine industry of China.

KEYWORDS:

new subgenotype; phylogenetic analysis; porcine reproductive and respiratory syndrome virus; recombinant strain

PMID:
28693303
PMCID:
PMC5799404
DOI:
10.4142/jvs.2018.19.1.89
[Indexed for MEDLINE]
Free PMC Article

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