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J Vet Sci. 2018 Mar 31;19(2):200-206. doi: 10.4142/jvs.2018.19.2.200.

Packaging of Rift Valley fever virus pseudoviruses and establishment of a neutralization assay method.

Li Y1,2,3, Zhao Y2, Wang C1,2, Zheng X2,4, Wang H2, Gai W2,5, Jin H6, Yan F2, Qiu B2,5, Gao Y2, Li N2, Yang S2, Xia X1,2.

Author information

1
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, China.
2
Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun 130122, China.
3
College of Animal Science and Technology, Shihezi University, Shihezi 832000, China.
4
School of Public Health, Shandong University, Jinan 250100, China.
5
College of Veterinary Medicine, Jilin University, Changchun 130012, China.
6
Changchun SR Biological Technology Co., Ltd., Changchun 130000, China.

Abstract

Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV). RVF is mainly prevalent on the Arabian Peninsula, the African continent, and several islands in the Indian Ocean near southeast Africa. RVFV has been classified by the World Organisation for Animal Health (OIE) as a category A pathogen. To avoid biological safety concerns associated with use of the pathogen in RVFV neutralization assays, the present study investigated and established an RVFV pseudovirus-based neutralization assay. This study used the human immunodeficiency virus (HIV) lentiviral packaging system and RVFV structural proteins to successfully construct RVFV pseudoviruses. Electron microscopy observation and western blotting indicated that the size, structure, and shape of the packaged pseudoviruses were notably similar to those of HIV lentiviral vectors. Infection inhibition assay results showed that an antibody against RVFV inhibited the infective ability of the RVFV pseudoviruses, and an antibody neutralization assay for RVFV detection was then established. This study has successfully established a neutralization assay based on RVFV pseudoviruses and demonstrated that this method can be used to effectively evaluate antibody neutralization.

KEYWORDS:

Rift Valley fever virus; antibody neutralization assay; pseudovirus

PMID:
28693302
PMCID:
PMC5879068
DOI:
10.4142/jvs.2018.19.2.200
[Indexed for MEDLINE]
Free PMC Article

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