Format

Send to

Choose Destination
Clin Oral Investig. 2018 Mar;22(2):929-940. doi: 10.1007/s00784-017-2172-5. Epub 2017 Jul 9.

The effect of saliva on the fate of nanoparticles.

Author information

1
Institute of Pharmaceutical Sciences, Department of Pharmaceutical Technology and Biopharmacy, University of Graz, Universitätsplatz 1, 8010, Graz, Austria.
2
BioTechMed, 8010, Graz, Austria.
3
Faculty of Mathematics and Physics, University of Ljubljana, 1000, Ljubljana, Slovenia.
4
Department of Nanobiomedicine, Mainz University Medical Center, 55131, Mainz, Germany.
5
Institute of Cell Biology, Histology and Embryology, Research Unit Electron Microscopic Techniques, Medical University of Graz, 8010, Graz, Austria.
6
Institute of Biophysics, Medical University of Graz, 8010, Graz, Austria.
7
Center for Medical Research, Medical University of Graz, 8010, Graz, Austria.
8
Institute for Process and Particle Engineering, Graz University of Technology, 8010, Graz, Austria.
9
Research Center Pharmaceutical Engineering, 8010, Graz, Austria.
10
Institute of Pharmaceutical Sciences, Department of Pharmaceutical Technology and Biopharmacy, University of Graz, Universitätsplatz 1, 8010, Graz, Austria. eva.roblegg@uni-graz.at.
11
BioTechMed, 8010, Graz, Austria. eva.roblegg@uni-graz.at.
12
Research Center Pharmaceutical Engineering, 8010, Graz, Austria. eva.roblegg@uni-graz.at.

Abstract

OBJECTIVES:

The design of nanocarriers for local drug administration to the lining mucosa requires a sound knowledge of how nanoparticles (NPs) interact with saliva. This contact determines whether NPs agglomerate and become immobile due to size- and interaction-filtering effects or adsorb on the cell surface and are internalized by epithelial cells. The aim of this study was to examine the behavior of NPs in saliva considering physicochemical NP properties.

MATERIALS AND METHODS:

The salivary pore-size distribution was determined, and the viscosity of the fluid inside of the pores was studied with optical tweezers. Distinct functionalized NPs (20 and 200 nm) were dispersed in saliva and salivary buffers and characterized, and surface-bound MUC5B and MUC7 were analyzed by 1D electrophoresis and immunoblotting. NP mobility was recorded, and cellular uptake studies were performed with TR146 cells.

RESULTS:

The mode diameter of the salivary mesh pores is 0.7 μm with a peak width of 1.9 μm, and pores are filled with a low-viscosity fluid. The physicochemical properties of the NPs affected the colloidal stability and mobility: compared with non-functionalized particles, which did not agglomerate and showed a cellular uptake rate of 2.8%, functionalized particles were immobilized, which was correlated with agglomeration and increased binding to mucins.

CONCLUSION:

The present study showed that the salivary microstructure facilitates NP adsorption. However, NP size and surface functionalization determine the colloidal stability and cellular interactions.

CLINICAL RELEVANCE:

The sound knowledge of NP interactions with saliva enables the improvement of current treatment strategies for inflammatory oral diseases.

KEYWORDS:

Biological barrier; Mobility; Mucoglycoproteins; Nanoparticles; Saliva

PMID:
28691145
PMCID:
PMC5820401
DOI:
10.1007/s00784-017-2172-5
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center