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J Biosci Bioeng. 2017 Nov;124(5):542-550. doi: 10.1016/j.jbiosc.2017.05.011. Epub 2017 Jul 6.

Probiotic-mediated blueberry (Vaccinium corymbosum L.) fruit fermentation to yield functionalized products for augmented antibacterial and antioxidant activity.

Author information

1
Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 54596, South Korea; Plant Medical Research Centre, College of Agricultural and Life Sciences, Chonbuk National University, Jenoju, Jeonbuk 54896, South Korea.
2
Microbial Institute for Fermentation Industry (MIFI), Sunchang, Jeonbuk 56048, South Korea.
3
Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 54596, South Korea.
4
Microbial Institute for Fermentation Industry (MIFI), Sunchang, Jeonbuk 56048, South Korea. Electronic address: jdy2534korea@gmail.com.

Abstract

The aim of this study was to investigate the fermentation of blueberry fruit with selected probiotic bacteria (Bacillus amyloliquefaciens and Lactobacillus brevis) and yeast (Starmerella bombicola) isolated from fermented starfish for the extraction of functionalized products for biomedical applications. All probiotic-based fermented extracts showed augmented antibacterial and antioxidant activity compared to the control. Biochemical parameters of viable cell count, titratable acidity, total phenol, total anthocyanin, total flavonoids, total sugar, and reducing sugar were analyzed during a 0-96 h fermentation period. In addition, Fourier transform infrared (FTIR) spectroscopy was performed to determine the functional groups in the control and fermented extracts and it signifies the presence of alcohol groups, phenol groups, carboxylic acids, and aliphatic amines, respectively. The well diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays determined that the S. bombicola-mediated fermented extract has excellent activity, followed by B. amyloliquefaciens and L. brevis, at a high concentration of 1.0 g/mL fermented extract. The ABTS and DPPH showed significant scavenging activity with IC50 values of (30.52 ± 0.08)/(155.10 ± 0.06) μg/mL, (24.82 ± 0.16)/(74.21 ± 1.26) μg/mL, and (21.81 ± 0.08)/(125.11 ± 0.04) μg/mL for B. amyloliquefaciens, L. brevis, and S. bombicola, respectively. Developing a value-added fermented blueberry product will help circumvent losses because of the highly perishable nature of the fruit.

KEYWORDS:

Antioxidant activity; Blueberry; Fermentation; Functional compound; Probiotics; Skin bacteria

PMID:
28690159
DOI:
10.1016/j.jbiosc.2017.05.011
[Indexed for MEDLINE]

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