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J Ethnopharmacol. 2017 Aug 17;208:72-83. doi: 10.1016/j.jep.2017.07.001. Epub 2017 Jul 5.

Antrodia camphorata inhibits metastasis and epithelial-to-mesenchymal transition via the modulation of claudin-1 and Wnt/β-catenin signaling pathways in human colon cancer cells.

Author information

1
Department of Cosmeceutics, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung 40402, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, Taichung 41354, Taiwan.
2
Institute of Nutrition, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung 40402, Taiwan.
3
Department of Medical Research, Chi-Mei Medical Center, Tainan 710, Taiwan.
4
Department of Life Sciences, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung 40402, Taiwan.
5
School of Pharmacy, College of Pharmacy, China Medical University, Taichung 40402, Taiwan.
6
Department of Cosmeceutics, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung 40402, Taiwan. Electronic address: varadha86@gmail.com.
7
Institute of Nutrition, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung 40402, Taiwan. Electronic address: hlyang@mail.cmu.edu.tw.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Antrodia camphorata (AC) is a well known traditional Chinese medicinal mushroom in Taiwan, has been used to treat various diseases including cancer.

MATERIALS AND METHODS:

In this study, we investigated the anti-metastatic and anti-EMT properties of a fermented culture broth of AC in human colon SW480claudin-1- and metastatic SW620claudin-1+ cancer cells in vitro.

RESULTS:

AC down-regulates claudin-1 and inhibits the proliferation and colony-formation abilities of both SW620claudin-1+ and SW480claudin-1- cells. In highly metastatic SW620claudin-1+ cells, non-cytotoxic concentrations of AC significantly inhibited migration/invasion, accompanied by the down-regulation of MMP-2 and MMP-9 proteins. AC decreased nuclear translocation of Wnt/β-catenin through a GSK3β-dependent pathway. AC consistently inhibited EMT by up-regulating the epithelial and downregulating the mesenchymal marker proteins. In SW480claudin-1- cells, AC suppressed migration/invasion potentially through the inhibition of the PI3K/AKT/NFκB signaling pathways without altering the expression levels of β-catenin and GSK3β proteins.

CONCLUSION:

Altogether, this study demonstrates the anti-metastatic and anti-EMT activities of AC, which may contribute to the development of a chemopreventive agent for colon cancer.

KEYWORDS:

Anti-EMT; Anti-metastatic; Antrodia camphorata; Chemopreventive; Colon cancer

PMID:
28688953
DOI:
10.1016/j.jep.2017.07.001
[Indexed for MEDLINE]

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