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Sci Rep. 2017 Jul 7;7(1):4895. doi: 10.1038/s41598-017-05155-w.

Integrated analysis of tobacco miRNA and mRNA expression profiles under PVY infection provids insight into tobacco-PVY interactions.

Author information

1
Key Laboratory of Molecular Genetics, China National Tobacco Corporation, Guizhou Institute of Tobacco Science, Guiyang, Guizhou, 550083, P. R. China.
2
Key Laboratory of Molecular Genetics, China National Tobacco Corporation, Guizhou Institute of Tobacco Science, Guiyang, Guizhou, 550083, P. R. China. jiamengao@cau.edu.cn.
3
Annoroad Gene Technology (Beijing) Co., Ltd, Beijing, 101100, P. R. China.
4
College of Agriculture and Food Science, Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang, 311300, P. R. China.
5
School of Life and Environmental science, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, P. R. China.
6
Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, 310058, P. R. China. drimran@zju.edu.cn.

Abstract

Potato virus Y (PVY) is a globally and economically important pathogen of potato, tobacco, tomato and other staple crops and caused significant yield losses and reductions in quality.To explore the molecular PVY-host interactions, we analysed changes in the miRNA and mRNA profiles of tobacco in response to PVY infection. A total of 81 differentially expressed miRNAs belonging to 29 families and 8133 mRNAs were identified. The Gene Ontology (GO) enrichment analyses showed that genes encoding the DNA/RNA binding, catalytic activity and signalling molecules were all significantly enriched. Moreover, 88 miRNA-mRNA interaction pairs were identified through a combined analysis of the two datasets. We also found evidence showing that the virus-derived siRNAs (vsiRNAs) from the PVY genome target tobacco translationally controlled tumor protein (NtTCTP) mRNA and mediate plant resistance to PVY. Together, our findings revealed that both miRNA and mRNA expression patterns can be changed in response to PVY infection and novel vsiRNA-plant interactions that may regulate plant resistance to PVY. Both provide fresh insights into the virus-plant interactions.

PMID:
28687775
PMCID:
PMC5501784
DOI:
10.1038/s41598-017-05155-w
[Indexed for MEDLINE]
Free PMC Article

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