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Dev Comp Immunol. 2017 Nov;76:334-342. doi: 10.1016/j.dci.2017.07.001. Epub 2017 Jul 3.

Chicken DNA virus sensor DDX41 activates IFN-β signaling pathway dependent on STING.

Author information

1
Shanghai Key Laboratory of Veterinary Biotechnology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
2
Shanghai Zoo, 2381 Hongqiao Road, Shanghai 200335, China.
3
The Center for Disease Prevention and Control of Baoshan, Shanghai 201901, China.
4
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
5
Shanghai Key Laboratory of Veterinary Biotechnology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: sunjhe@sjtu.edu.cn.

Abstract

The recognition of pathogenic DNA is important to the initiation of antiviral responses. Here, we report the identification of the first avian DEAD (Asp-Glu-Ala-Asp) box polypeptide 41 (DDX41), an important DNA sensor, in chicken cells. In our study, we confirmed that chDDX41 is not an interferon-inducible gene. Knockdown of chDDX41 expression by shRNA blocked the ability of DF-1 cells to mount an IFN-β response to DNA and associated viruses. ChDDX41 mRNAs could be upregulated by double-stranded DNA (dsDNA) analogue poly(dA:dT), but not by double-stranded RNA (dsRNA) analogue poly(I:C). In poly(dA:dT) stimulation assays, the immune molecules involved in the DDX41-mediated IFN-β pathway in human cells were universally upregulated in chicken cells. Via coimmunoprecipitation (Co-IP) experiments, we found that chDDX41 could strongly interact with chicken stimulator of IFN genes (chSTING). Therefore, our results suggest that chDDX41 is involved in the dsDNA- and dsDNA virus-mediated chDDX41-chSTING-IFN-β signaling pathway in chicken cells.

KEYWORDS:

Chicken; DDX41; DNA sensor; DNA virus; IFN-β; STING

PMID:
28684273
DOI:
10.1016/j.dci.2017.07.001
[Indexed for MEDLINE]

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