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Indian J Med Microbiol. 2017 Apr-Jun;35(2):302-304. doi: 10.4103/ijmm.IJMM_15_333.

Development and evaluation of a loop-mediated isothermal amplification combined with au-nanoprobe assay for rapid detection of Mycobacterium tuberculosis.

Author information

1
Innovative Learning Center, Srinakharinwirot University, Bangkok 10110, Thailand.
2
Department of Clinical Pathology, Faculty of Medicine, Vajira Hospital, Navamindradhiraj University, Bangkok 10110, Thailand.
3
Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand.
4
Department of Pathology, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand.

Abstract

Loop-mediated isothermal amplification (LAMP) has been proposed as an inexpensive and easy to perform assay for molecular diagnostics. We present a novel strategy for the detection of LAMP amplicons derived from Mycobacterium tuberculosis by the use of Au-nanoprobes. When applied to a total of 93 clinical specimens, the LAMP assay demonstrated sensitivity and specificity higher than that of polymerase chain reaction and culture. The Au-nanoprobe augmented LAMP test platform with its advantages of robust reagents and a simple colorimetric detection method can be adapted easily for the rapid detection of other infectious disease agents at a low cost.

PMID:
28681828
DOI:
10.4103/ijmm.IJMM_15_333
[Indexed for MEDLINE]
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