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Immune Netw. 2017 Jun;17(3):163-170. doi: 10.4110/in.2017.17.3.163. Epub 2017 Jun 20.

Clonal Expansion of Allergen-specific CD4+ T Cell in the Lung in the Absence of Lymph Nodes.

Author information

1
Laboratory of Immune Regulation, Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Korea.
2
BK21 Plus Program, College of Pharmacy, Seoul National University, Seoul 08826, Korea.

Abstract

The expansion of allergen-specific CD4+ T cells is a critical step in inducing airway inflammation during allergic asthma. Such clonal expansion of T cells is initiated through the interaction between allergen-bearing dendritic cells and allergen-specific naïve T cells in the draining lymph nodes. Whether such T cell clonal expansion also occurs in the lung, the primary organ encountering inhaled allergens, remains unclear. Compared with wild-type mice, we found similar frequencies of CD4+ T cells in the lung of lymph node-deficient Rorgtgfp/gfp mice after repeated exposure to inhaled allergens. In addition, we observed an evident population of CD4+ T cells that underwent clonal expansion in the lung of allergen-challenged mice treated with an S1P antagonist FTY720 in an in vivo proliferation study with CFSE-labeled OT-II T cells. Moreover, the expansion of allergen-specific CD4+ T cells was significantly enhanced in the lungs of Rorgtgfp/gfp mice in comparison to that of wild-type mice. These results together demonstrate that the clonal expansion of allergen-specific CD4+ T cells occurs in the absence of the lymph nodes, indicating that the lung can act as a primary site of the clonal expansion of CD4+ T cells in response to inhaled allergens.

KEYWORDS:

Allergens; CD4+ T cell; Lung; Proliferation; RORγt

Conflict of interest statement

CONFLICTS OF INTEREST: The authors have no financial conflict of interest.

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