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Cent Eur J Immunol. 2017;42(1):17-23. doi: 10.5114/ceji.2017.67314. Epub 2017 May 8.

Different effects of feeding pregnant and lactating mice Rhodiola kirilowii aqueous and hydro-alcoholic extracts on their serum angiogenic activity and content of selected polyphenols.

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Department of Regenerative Medicine and Cell Biology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
Department of Microwave Safety, Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
Pathomorphology Department, Center for Biostructure Research, Warsaw Medical University, Warsaw, Poland.
Department of Physiological Sciences Faculty of Veterinary Medicine Warsaw University of Life Sciences, Warsaw, Poland.
Laboratory of Parasitology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
Laboratory of Food and Nutrition Hygiene, Military Institute of Hygiene and Epidemiology, Warsaw, Poland.


Angiogenesis plays an important role in many physiological processes, among them the formation of tissues and organs during embryogenesis. A lot of medicinal plants exhibit angiomodulatory properties. This creates the need for a thorough check of whether the plant extracts that we would like to give to pregnant women in order to increase their resistance to bacterial or viral infection will have negative effects on angiogenesis, and consequently on fetal development. This paper seeks to investigate the effect of serum of pregnant and nursing Balb/c mice that received aqueous (RKW) or hydro-alcoholic (RKW-A) R. kirilowii extracts (20 mg/kg), or epigallocatechin (0.2 mg/kg), on the in vitro proliferation and migration of mouse endothelial cell line Heca10. Of the 15 identified polyphenols in the extracts by HPLC, 8 were present in the sera. Chemical analysis revealed higher salidroside, kaempferol, chlorogenic acid, bFGF and VEGF concentration in RKW-A sera than in the sera of RKW group of mice. RKW-A and EGC sera did not affect migration of endothelial cells, however we noted some increase of migrating cells after RKW-sera treatment. RKW and EGC sera did not affect proliferation of endothelial cells. Sera of mothers from RKW-A group impaired the proliferation of endothelial cells in comparison to other groups. These data allow us to assume that Rhodiola kirilowii hydro-alcoholic extract (RKW-A) is potentially able to modulate pre- and post- natal angiogenesis what might influence the development of organs in progeny. Sera of RKW mothers have not harm the proliferation of endothelial cells, despite they also contain antiangiogenic catechins and salidroside. This suggests the existence in RKW-A extract and in RKW-A sera of some other, as yet unidentified substances influencing endothelial cells proliferation.


Rhodiola kirilowii; angiogenesis; mice; polyphenols; pregnancy

Conflict of interest statement

The authors declare no conflict of interest.

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