Format

Send to

Choose Destination
Sci Rep. 2017 Jul 5;7(1):4699. doi: 10.1038/s41598-017-04740-3.

Challenging the Wigglesworthia, Sodalis, Wolbachia symbiosis dogma in tsetse flies: Spiroplasma is present in both laboratory and natural populations.

Author information

1
Department of Environmental and Natural Resources Management, University of Patras, Agrinio, 30100, Greece.
2
Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK.
3
Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna International Centre, P.O. Box 100, 1400, Vienna, Austria.
4
Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 5QA, UK.
5
School of Environment and Life Sciences, The Crescent, University of Salford, Salford, M5 4WT, UK.
6
Institut de Recherche pour le Développement, Unité Mixte de Recherche Interactions Hôtes-Vecteurs-Parasites-Environnement dans les Maladies Tropicales Négligées Dues aux Trypanosomatides, 34398, Montpellier, France.
7
Centre International de Recherche-Développement sur l'Élevage en zone Subhumide (CIRDES), N°559, Rue 5-31 Avenue du Gouverneur Louveau, 01 BP 454, Bobo Dioulasso 01, Burkina Faso.
8
Institute of Zoology, Section of Molecular and Applied Zoology, Slovak Academy of Sciences, Dubravska cesta 9, 845 06, Bratislava, Slovakia.
9
Scientica, Ltd, Hybesova 33, 831 06, Bratislava, Slovakia.
10
National Institute for Control and Eradication of Tsetse and Trypanosomosis (NICETT), Kality Centre, Akaki Kality, Addis Ababa, Ethiopia.
11
Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK. Alistair.Darby@liverpool.ac.uk.
12
Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna International Centre, P.O. Box 100, 1400, Vienna, Austria. K.Bourtzis@iaea.org.
13
Department of Environmental and Natural Resources Management, University of Patras, Agrinio, 30100, Greece. gtsiamis@upatras.gr.

Abstract

Profiling of wild and laboratory tsetse populations using 16S rRNA gene amplicon sequencing allowed us to examine whether the "Wigglesworthia-Sodalis-Wolbachia dogma" operates across species and populations. The most abundant taxa, in wild and laboratory populations, were Wigglesworthia (the primary endosymbiont), Sodalis and Wolbachia as previously characterized. The species richness of the microbiota was greater in wild than laboratory populations. Spiroplasma was identified as a new symbiont exclusively in Glossina fuscipes fuscipes and G. tachinoides, members of the palpalis sub-group, and the infection prevalence in several laboratory and natural populations was surveyed. Multi locus sequencing typing (MLST) analysis identified two strains of tsetse-associated Spiroplasma, present in G. f. fuscipes and G. tachinoides. Spiroplasma density in G. f. fuscipes larva guts was significantly higher than in guts from teneral and 15-day old male and female adults. In gonads of teneral and 15-day old insects, Spiroplasma density was higher in testes than ovaries, and was significantly higher density in live versus prematurely deceased females indicating a potentially mutualistic association. Higher Spiroplasma density in testes than in ovaries was also detected by fluorescent in situ hybridization in G. f. fuscipes.

PMID:
28680117
PMCID:
PMC5498494
DOI:
10.1038/s41598-017-04740-3
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center