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Cold Spring Harb Protoc. 2017 Jul 5;2017(7):pdb.prot089003. doi: 10.1101/pdb.prot089003.

Chemostat Culture for Yeast Physiology.

Author information

1
Department of Genome Sciences, University of Washington, Seattle, Washington 98195.
2
Department of Genome Sciences, University of Washington, Seattle, Washington 98195 maitreya@uw.edu.

Abstract

The use of chemostat culture facilitates the careful comparison of different yeast strains growing in well-defined conditions. Variations in physiology can be measured by examining gene expression, metabolite levels, protein content, and cell morphology. In this protocol, we show how a combination of sample types can be collected during harvest from a single 20-mL chemostat in a ministat array, with special attention to coordinating the handling of the most time-sensitive sample types.

PMID:
28679699
DOI:
10.1101/pdb.prot089003
[Indexed for MEDLINE]

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