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Cold Spring Harb Protoc. 2017 Jul 5;2017(7):pdb.prot088997. doi: 10.1101/pdb.prot088997.

Assembly of a Mini-Chemostat Array.

Author information

1
Department of Genome Sciences, University of Washington, Seattle, Washington 98195.
2
Department of Genome Sciences, University of Washington, Seattle, Washington 98195 maitreya@uw.edu.

Abstract

Here, we describe instructions for the assembly of an array of miniature (20-mL) chemostats or "ministats" built from relatively inexpensive off-the-shelf parts. In experiments with yeast cultures, we have observed reproducibility in cellular physiology, gene expression patterns, and evolutionary outcomes with different ministats as well as between ministats and commercial large-volume platforms. Growth in continuous culture is a primary means for the characterization of yeast steady-state physiology, competition between strains, and long-term evolution experiments. We hope that these relatively inexpensive and high-throughput devices make the advantages of continuous culture growth more accessible to researchers.

PMID:
28679698
DOI:
10.1101/pdb.prot088997
[Indexed for MEDLINE]

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