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Sci Rep. 2017 Jul 4;7(1):4607. doi: 10.1038/s41598-017-04869-1.

Selective breeding and selection mapping using a novel wild-derived heterogeneous stock of mice revealed two closely-linked loci for tameness.

Author information

1
Mouse Genomics Resource Laboratory, National Institute of Genetics, Yata, Mishima, Shizuoka, 411-8540, Japan.
2
Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies), Yata, Mishima, Shizuoka, 411-8540, Japan.
3
Graduate School of Medicine, Nagoya University, Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
4
Division of Human Genetics, National Institute of Genetics, Yata, Mishima, Shizuoka, 411-8540, Japan.
5
Transdisciplinary Research Integration Center, Toranomon, Minatoku, Tokyo, 105-0001, Japan.
6
Drosophila Genetic Resource Center, Kyoto Institute of Technology, Kyoto, 616-8354, Japan.
7
Genetics Institute, University College London, Gower Street, London, WC1E 6BT, UK.
8
Mouse Genomics Resource Laboratory, National Institute of Genetics, Yata, Mishima, Shizuoka, 411-8540, Japan. tkoide@nig.ac.jp.
9
Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies), Yata, Mishima, Shizuoka, 411-8540, Japan. tkoide@nig.ac.jp.
10
Transdisciplinary Research Integration Center, Toranomon, Minatoku, Tokyo, 105-0001, Japan. tkoide@nig.ac.jp.

Abstract

Tameness is a major behavioral factor for domestication, and can be divided into two potential components: motivation to approach humans (active tameness) and reluctance to avoid humans (passive tameness). We identified genetic loci for active tameness through selective breeding, selection mapping, and association analysis. In previous work using laboratory and wild mouse strains, we found that laboratory strains were predominantly selected for passive tameness but not active tameness during their domestication. To identify genetic regions associated with active tameness, we applied selective breeding over 9 generations for contacting, a behavioural parameter strongly associated with active tameness. The prerequisite for successful selective breeding is high genetic variation in the target population, so we established and used a novel resource, wild-derived heterogeneous stock (WHS) mice from eight wild strains. The mice had genetic variations not present in other outbred mouse populations. Selective breeding of the WHS mice increased the contacting level through the generations. Selection mapping was applied to the selected population using a simulation based on a non-selection model and inferred haplotype data derived from single-nucleotide polymorphisms. We found a genomic signature for selection on chromosome 11 containing two closely linked loci.

PMID:
28676693
PMCID:
PMC5496859
DOI:
10.1038/s41598-017-04869-1
[Indexed for MEDLINE]
Free PMC Article

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