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Methods Mol Biol. 2017;1619:203-212. doi: 10.1007/978-1-4939-7057-5_16.

Lipidomics of Human Blood Plasma by High-Resolution Shotgun Mass Spectrometry.

Author information

1
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307, Dresden, Germany.
2
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307, Dresden, Germany. shevchenko@mpi-cbg.de.

Abstract

Clinical lipidomics is an emerging biomarker discovery approach that compares lipid profiles under pathologically and physiologically normal conditions. Here we describe a method for the absolute (molar) quantification of more than 200 molecules from 14 major lipid classes from 5 μL of human blood plasma using high-resolution top-down shotgun mass spectrometry. Because of its technical simplicity and robustness, the protocol lends itself for high-throughput clinical lipidomics screens.

KEYWORDS:

Blood plasma; LipidXplorer; Lipids; Mass spectrometry; Shotgun lipidomics

PMID:
28674888
DOI:
10.1007/978-1-4939-7057-5_16
[Indexed for MEDLINE]

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