Format

Send to

Choose Destination
Brief Bioinform. 2017 Jun 30. doi: 10.1093/bib/bbx067. [Epub ahead of print]

Alignment-free inference of hierarchical and reticulate phylogenomic relationships.

Abstract

We are amidst an ongoing flood of sequence data arising from the application of high-throughput technologies, and a concomitant fundamental revision in our understanding of how genomes evolve individually and within the biosphere. Workflows for phylogenomic inference must accommodate data that are not only much larger than before, but often more error prone and perhaps misassembled, or not assembled in the first place. Moreover, genomes of microbes, viruses and plasmids evolve not only by tree-like descent with modification but also by incorporating stretches of exogenous DNA. Thus, next-generation phylogenomics must address computational scalability while rethinking the nature of orthogroups, the alignment of multiple sequences and the inference and comparison of trees. New phylogenomic workflows have begun to take shape based on so-called alignment-free (AF) approaches. Here, we review the conceptual foundations of AF phylogenetics for the hierarchical (vertical) and reticulate (lateral) components of genome evolution, focusing on methods based on k-mers. We reflect on what seems to be successful, and on where further development is needed.

KEYWORDS:

D2 statistics; TF–IDF; alignment-free; k-mer; lateral genetic transfer; phylogenomics

PMID:
28673025
DOI:
10.1093/bib/bbx067

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center