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Plant J. 2017 Oct;92(1):19-30. doi: 10.1111/tpj.13630. Epub 2017 Aug 21.

Temporal and spatial transcriptomic and microRNA dynamics of CAM photosynthesis in pineapple.

Author information

1
FAFU and UIUC Joint Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Key Laboratory of Genetics, Breeding and Multiple Utilization of Corps, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
2
Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
3
Donald Danforth Plant Science Center, St. Louis, MO, USA.
4
Department of Horticulture, Michigan State University, East Lansing, MI, 48823, USA.
5
Department of Biochemistry and Molecular Biology, MS330, University of Nevada, Reno, NV, 89557-0330, USA.
6
Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK.

Abstract

The altered carbon assimilation pathway of crassulacean acid metabolism (CAM) photosynthesis results in an up to 80% higher water-use efficiency than C3 photosynthesis in plants making it a potentially useful pathway for engineering crop plants with improved drought tolerance. Here we surveyed detailed temporal (diel time course) and spatial (across a leaf gradient) gene and microRNA (miRNA) expression patterns in the obligate CAM plant pineapple [Ananas comosus (L.) Merr.]. The high-resolution transcriptome atlas allowed us to distinguish between CAM-related and non-CAM gene copies. A differential gene co-expression network across green and white leaf diel datasets identified genes with circadian oscillation, CAM-related functions, and source-sink relations. Gene co-expression clusters containing CAM pathway genes are enriched with clock-associated cis-elements, suggesting circadian regulation of CAM. About 20% of pineapple microRNAs have diel expression patterns, with several that target key CAM-related genes. Expression and physiology data provide a model for CAM-specific carbohydrate flux and long-distance hexose transport. Together these resources provide a list of candidate genes for targeted engineering of CAM into C3 photosynthesis crop species.

KEYWORDS:

Ananas comosus ; CAM photosynthesis; circadian regulation; cis-element; stomata

PMID:
28670834
DOI:
10.1111/tpj.13630
[Indexed for MEDLINE]
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