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Bio Protoc. 2017 Apr 5;7(7). pii: e2213. doi: 10.21769/BioProtoc.2213.

In vitro Detection of Neutrophil Traps and Post-attack Cell Wall Changes in Candida Hyphae.

Author information

1
Molecular and Biomedical Sciences, University of Maine, Orono, Maine, USA.
2
Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono, Maine, USA.

Abstract

In this protocol we describe how to visualize neutrophil extracellular traps (NETs) and fungal cell wall changes in the context of the coculture of mouse neutrophils with fungal hyphae of Candida albicans. These protocols are easily adjusted to test a wide array of hypotheses related to the impact of immune cells on fungi and the cell wall, making them promising tools for exploring host-pathogen interactions during fungal infection.

KEYWORDS:

C. albicans; Cell wall; Fungi; Host-pathogen interactions; NETs; sDectin-1-Fc

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