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Biochem Biophys Res Commun. 2017 Sep 23;491(3):807-813. doi: 10.1016/j.bbrc.2017.06.184. Epub 2017 Jun 30.

Mangiferin protects osteoblast against oxidative damage by modulation of ERK5/Nrf2 signaling.

Author information

1
Department of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan, China.
2
Department of Ultrasound, Cardiovascular Disease and Cerebrovascular Disease Hospital, General Hospital of Ningxia Medical University, Yinchuan, China.
3
Department of General Surgery, The Third People's Hospital of Ningxia, Yinchuan, China.
4
Department of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan, China. Electronic address: yongdongqiaoq@163.com.

Abstract

Oxidative stress has currently been proposed as a risk factor associated with the development and proression of osteoporosis. In this study, we identify the effect of mangiferin (MAN) on apoptosis and differentiation of osteoblast-like MC3T3-E1 cells insulted by H2O2. We firstly found that MAN can promote cell proliferation of MC3T3-E1 cells in a time- and dose-dependent manner and stimulate the phosphorylation of ERK5. Cells were divided as five groups: control, H2O2 (100 μM, control), H2O2 + MAN (5 μM), H2O2 + MAN (10 μM), and H2O2 + MAN (20 μM). MAN can significantly decrease H2O2-induced apoptosis and elevated ROS level of MC3T3-E1 cells. The expressions of caspase-3, caspase-9 and Bax/Bcl-2 were increased with H2O2 treatment, and MAN can reverse these changes. In addition, Nrf2 and its downstream target effectors (HO1, NQO1) were dramatically attenuated in MC3T3-E cells treatment with H2O2, while MAN can significantly increase the expression of Nrf2, HO1 and NQO1. The expression of ERK5 was down regulated by RNA interference in MC3T3-E1 cells, and we found that MAN (20 μM) pretreatment didn't make remarkable decrease in cell apoptosis or expressions of apoptosis-related proteins in H2O2-insulted siRNA-ERK5 cells. This study indicated that MAN can protect osteoblast against oxidative damage by modulation of ERK5/Nrf2 signaling, which can be new agent for osteoporosis.

KEYWORDS:

ERK5; MAN; Nrf2; Osteoblast; Oxidative damage

PMID:
28669729
DOI:
10.1016/j.bbrc.2017.06.184
[Indexed for MEDLINE]

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