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Asian-Australas J Anim Sci. 2017 Jul;30(7):950-956. doi: 10.5713/ajas.17.0287. Epub 2017 Jun 22.

Development of a new lactic acid bacterial inoculant for fresh rice straw silage.

Author information

1
Graduate School of International Agricultural Technology and Institute of Green Bio Science & Technology, Seoul National University, Pyeongchang 25354, Korea.
2
Animal Products Research and Development Division, National Institute of Animal Science, Wanju 55365, Korea.
3
Grassland and Forage Division, National Institute of Animal Science, Cheonan 31000, Korea.

Abstract

OBJECTIVE:

Effects of newly isolated Lactobacillus plantarum on the fermentation and chemical composition of fresh rice straw silage was evaluated in this study.

METHODS:

Lactic acid bacteria (LAB) from good crop silage were screened by growing them in MRS broth and a minimal medium with low carbohydrate content. Selected LAB (LAB 1821) were Gram-positive, rods, catalase negative, and were identified to be Lactobacillus plantarum based on their biochemical characteristics and a 16S rRNA analysis. Fresh rice straw was ensiled with two isolated LAB (1821 and 1841), two commercial inoculants (HM/F and P1132) and no additive as a control.

RESULTS:

After 2 months of storage at ambient temperature, rice straw silages treated with additives were well-preserved, the pH values and butyric and acetic acid contents were lower, and the lactic acid content and lactic/acetic acid ratio were higher than those in the control (p<0.05). Acidity (pH) was lowest, and lactic acid highest, in 1821-treated silage (p<0.05). The NH3-N content decreased significantly in inoculant-treated silage (p<0.05) and the NH3-N content in 1821-treated silage was lowest among the treatments. The dry matter (DM) content of the control silage was lower than that of fresh rice straw (p<0.05), while that of the 1841- and p1174-inoculant-treated silages was significantly higher than that of HM/F-treated silage. Microbial additives did not have any significant (p>0.05) effect on acid detergent fiber or neutral detergent fiber contents. Crude protein (CP) content and in vitro DM digestibility (IVDMD) increased after inoculation of LAB 1821 (p<0.05).

CONCLUSION:

LAB 1821 increased the CP, IVDMD, lactic acid content and ratio of lactic acid to acetic acid in rice straw silage and decreased the pH, acetic acid, NH3-N, and butyric acid contents. Therefore, adding LAB 1821 improved the fermentation quality and feed value of rice straw silage.

KEYWORDS:

Fermentation; Inoculant; Lactic Acid Bacteria; Quality; Rice Straw Silage

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