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Anticancer Res. 2017 Jul;37(7):3917-3920.

Lipopolysaccharides Derived from Pantoea agglomerans Can Promote the Phagocytic Activity of Amyloid β in Mouse Microglial Cells.

Author information

1
Department of Integrated and Holistic Immunology, Faculty of Medicine, Kagawa University, Kagawa, Japan kobayashi@shizenmeneki.org.
2
Department of Integrated and Holistic Immunology, Faculty of Medicine, Kagawa University, Kagawa, Japan.
3
Control of Innate Immunity, Technology Research Association, Kagawa, Japan.
4
Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan.
5
Macrophi Inc., Takamatsu, Kagawa, Japan.
6
Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Kagawa, Japan.
7
Department of Gastroenterology and Neurology, Faculty of Medicine, Kagawa University, Kagawa, Japan.

Abstract

BACKGROUND/AIM:

Recent studies reported that lipopolysaccharide (LPS) exhibits beneficial effects on prevention of immune-related diseases by activating macrophages. We previously demonstrated that pre-treatment with LPS derived from Pantoea agglomerans (LPSp) activated amyloid β (Aβ) phagocytosis in mouse primary microglia. In the present study, we further examined the promotory effect on phagocytosis of phagocytic particles in the C8-B4 microglia cell line.

MATERIALS AND METHODS:

Phagocytic analysis of C8-B4 cells was evaluated using phagocytic particles (latex beads or HiLyte™ Fluor 488-conjugated Aβ1-42).

RESULTS:

The phagocytic activity of latex beads was dependent on the concentration of beads and incubation time. LPSp, at as low as 100 pg/ml, significantly increased phagocytosis against the beads. In the experiment of Aβ1-42 phagocytosis, LPSp significantly increased Aβ phagocytic activity.

CONCLUSION:

LPSp treatment was confirmed to enhance Aβ1-42 phagocytosis by mouse microglia. It is suggested that the use of LPSp may be a potential promising candidate for the prevention of Alzheimer's disease.

KEYWORDS:

Lipopolysaccharide; Pantoea agglomerans; amyloid β; microglia; toll-like receptor

PMID:
28668895
DOI:
10.21873/anticanres.11774
[Indexed for MEDLINE]

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