Format

Send to

Choose Destination
Anticancer Res. 2017 Jul;37(7):3521-3526.

Itraconazole Modulates Hedgehog, WNT/β-catenin, as well as Akt Signalling, and Inhibits Proliferation of Cervical Cancer Cells.

Author information

1
Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Japan.
2
Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Japan tsuba@hyo-med.ac.jp.
3
Department of Medical Oncology, Meiwa Hospital, Nishinomiya, Japan.

Abstract

BACKGROUND/AIM:

Repurposing itraconazole as an anticancer agent has been evaluated in several studies. The present study investigated whether itraconazole exerts an anticancer effect on cervical cancer cells.

MATERIALS AND METHODS:

CaSki and HeLa cells were cultured in itraconazole and vehicle after which colony-forming and cell viability assays were performed. Transcription and protein expression were assessed by cDNA microarray analysis and immunoblotting, respectively.

RESULTS:

Itraconazole suppressed proliferation of CaSki and HeLa cells in a dose- and time-dependent manner. Furthermore, CaSki cells were more significantly affected by itraconazole than HeLa cells. The microarray analysis showed an 8-fold down-regulation in the expression of GLI1, WNT4 and WNT10A among itraconazole-treated CaSki cells. Moreover, the transcription of sterol carrier protein-2 and ATP-binding cassette transporter-1 was unaffected by itraconazole. Immunoblots showed suppression in β-catenin expression and Akt phosphorylation.

CONCLUSION:

Itraconazole is a multi-targeting anticancer agent and a promising therapeutic agent for cervical cancer.

KEYWORDS:

AKT/mTOR; Itraconazole; WNT/β-catenin; cervical cancer; hedgehog

PMID:
28668841
DOI:
10.21873/anticanres.11720
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center