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Anticancer Res. 2017 Jul;37(7):3453-3459.

Effects of Pulsed Electromagnetic Fields on Breast Cancer Cell Line MCF 7 Using Absorption Spectroscopy.

Author information

1
Optics and Instrumentation Physics Laboratory, Physics Department, De La Salle University, Manila, Philippines.
2
Faculty of Science, Liverpool Hope University, Liverpool, U.K. naguibr@hope.ac.uk r.naguib@biocoreinternational.com.
3
BIOCORE Research & Consultancy International, Liverpool, U.K.

Abstract

We present an analysis of the effects of pulsed electromagnetic fields (PEMF) with 3.3 MHz carrier frequency and modulated by audio resonant frequencies on the MCF-7 breast cancer cell line in vitro using absorption spectroscopy. This involves a fluorescence dye called PrestoBlue™ Cell Viability Reagent and a spectrophotometry to test the viability of MCF-7 breast cancer cells under different PEMF treatment conditions in terms of the cell absorption values. The DNA molecule of the MCF-7 breast cancer cells has an electric dipole property that renders it sensitive and reactive to applied electromagnetic fields. Resonant frequencies derived from four genes mutated in MCF-7 breast cancer cells [rapamycin-insensitive companion of mammalian target of rapamycin (RICTOR), peroxisome proliferator-activated receptor (PPARG), Nijmegen breakage syndrome 1 (NBN) and checkpoint kinase 2 (CHEK2)] were applied in generating square pulsed electromagnetic waves. Effects were monitored through measurement of absorption of the samples with PrestoBlue™, and the significance of the treatment was determined using the t-test. There was a significant effect on MCF-7 cells after treatment with PEMF at the resonant frequencies of the following genes for specific durations of exposure: RICTOR for 10 min, PPARG for 10 min, NBN for 15 min, and CHEK2 for 5 min.

KEYWORDS:

Breast cancer; PrestoBlueT™; gene mutation; pulsed electromagnetic fields; spectroscopy

PMID:
28668834
DOI:
10.21873/anticanres.11713
[Indexed for MEDLINE]

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