Format

Send to

Choose Destination
Pharmacol Res. 2017 Sep;123:103-113. doi: 10.1016/j.phrs.2017.06.015. Epub 2017 Jun 28.

Isomalto-oligosaccharides, a prebiotic, functionally augment green tea effects against high fat diet-induced metabolic alterations via preventing gut dysbacteriosis in mice.

Author information

1
National Agri-Food Biotechnology Institute (NABI), SAS Nagar, Punjab, 140306, India; Pharmacology Division, University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Chandigarh,160014, India.
2
National Agri-Food Biotechnology Institute (NABI), SAS Nagar, Punjab, 140306, India.
3
Department of Biotechnology, Government College for Girls-Sector 42, Chandigarh, 160036, India.
4
BioNeutra North America Incorporation, 9608 25 Avenue NW, Edmonton, Alberta T6N 1E5, Canada.
5
Division of Endocrinology and Metabolism, The EVMS Sterling Centre of Diabetes and Endocrine Disorders, Department of Internal Medicine, East Virginia Medical School, 855 West Brambleton Avenue, Norfolk, VA 23510, USA.
6
National Agri-Food Biotechnology Institute (NABI), SAS Nagar, Punjab, 140306, India. Electronic address: kiran@nabi.res.in.
7
Pharmacology Division, University Institute of Pharmaceutical Sciences (UIPS), Panjab University, Chandigarh,160014, India. Electronic address: kanwaljitchopra@yahoo.co.in.
8
National Agri-Food Biotechnology Institute (NABI), SAS Nagar, Punjab, 140306, India. Electronic address: mbishnoi@gmail.com.

Abstract

High fat diet (HFD)-induced alterations in gut microbiota and resultant 'leaky gut' phenomenon promotes metabolic endotoxemia, ectopic fat deposition, and low-grade systemic inflammation. Here we evaluated the effects of a combination of green tea extract (GTE) with isomalto-oligosaccharide (IMOs) on HFD-induced alterations in mice. Male Swiss albino mice were fed with HFD (58% fat kcal) for 12 weeks. Systemic adiposity, gut derangement parameters and V3-V4 region based 16S rRNA metagenomic sequencing, ectopic fat deposition, liver metabolome analysis, systemic and tissue inflammation, and energy homeostasis markers along with gene expression analysis in multiple tissues were done in mice supplemented with GTE, IMOs or their combination. The combination of GTE and IMOs effectively prevented HFD-induced adiposity and lipid accumulation in liver and muscle while normalizing fasting blood glucose, insulin, glucagon, and leptin levels. Co-administration of GTE with IMOs effectively modulated liver metabolome associated with lipid metabolism. It also prevented leaky gut phenotype and HFD-induced increase in circulating lipopolysaccharides and pro-inflammatory cytokines (e.g. resistin, TNF-α, and IL-1β) and reduction in anti-inflammatory cytokines (e.g. adiponectin and IL-6). Gene expression analysis across multiple tissues further supported these functional outcomes. Most importantly, this combination improved beneficial gut microbiota (Lactobacillus sp., Bifidobacteria, Akkermansia muciniphila, Roseburia spp.) abundances, restored Firmicutes/Bacteriodetes and improved Prevotella/Bacteroides proportions. In particular, a combination of these two agents has shown improved beneficial effects on multiple parameters studied. Data presented herein suggests that strategically chosen food components might be highly effective in the prevention of HFD-induced alterations and may further be developed as functional foods.

KEYWORDS:

Gut dysbacteriosis; High fat diet; Inflammation; Insulin resistance; Liver metabolites; Metabolic endotoxemia

PMID:
28668709
DOI:
10.1016/j.phrs.2017.06.015
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center