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Methods. 2017 Aug 1;125:63-69. doi: 10.1016/j.ymeth.2017.06.028. Epub 2017 Jun 29.

Studying structure and function of spliceosomal helicases.

Author information

1
Department of Molecular Structural Biology, Institute of Microbiology and Genetics, GZMB, Georg-August-University Göttingen, 37077 Göttingen, Germany. Electronic address: rficner@uni-goettingen.de.
2
Department of Molecular Structural Biology, Institute of Microbiology and Genetics, GZMB, Georg-August-University Göttingen, 37077 Göttingen, Germany.

Abstract

The splicing of eukaryotic precursor mRNAs requires the activity of at least three DEAD-box helicases, one Ski2-like helicase and four DEAH-box helicases. High resolution structures for five of these spliceosomal helicases were obtained by means of X-ray crystallography. Additional low resolution structural information could be derived from single particle cryo electron microscopy and small angle X-ray scattering. The functional characterization includes biochemical methods to measure the ATPase and helicase activities. This review gives an overview on the techniques used to gain insights in to the structure and function of spliceosomal helicases.

PMID:
28668587
DOI:
10.1016/j.ymeth.2017.06.028
[Indexed for MEDLINE]

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