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Methods Mol Biol. 2017;1615:151-158. doi: 10.1007/978-1-4939-7033-9_12.

Measure of Peptidoglycan Hydrolase Activity.

Author information

1
Laboratoire d'Ingénierie des Systèmes Macromoléculaires (LISM, UMR 7255), Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ-CNRS, 31 Chemin Joseph Aiguier, 13402, Marseille Cedex 20, France.
2
Laboratoire d'Ingénierie des Systèmes Macromoléculaires (LISM, UMR 7255), Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ-CNRS, 31 Chemin Joseph Aiguier, 13402, Marseille Cedex 20, France. cascales@imm.cnrs.fr.

Abstract

Most gene clusters encoding multiprotein complexes of the bacterial cell envelope, such as conjugation and secretion systems, Type IV pili, and flagella, bear a gene encoding an enzyme with peptidoglycan hydrolase activity. These enzymes are usually glycoside hydrolases that cleave the glycan chains of the peptidoglycan. Their activities are spatially controlled to avoid cell lysis and to create localized rearrangement of the cell wall. This is assured by interaction with the structural subunits of the apparatus. Here we describe protocols to test the peptidoglycan hydrolase activity of these proteins in vitro and in solution.

KEYWORDS:

Cell wall; Localized degradation; Lytic transglycosylase; Peptidoglycan; Remazol blue

PMID:
28667610
DOI:
10.1007/978-1-4939-7033-9_12
[Indexed for MEDLINE]

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