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Environ Sci Pollut Res Int. 2017 Aug;24(23):19267-19281. doi: 10.1007/s11356-017-9438-y. Epub 2017 Jun 30.

Effects of low doses of glyphosate on DNA damage, cell proliferation and oxidative stress in the HepG2 cell line.

Author information

1
Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia.
2
Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia. mmilic@imi.hr.
3
Xellia Ltd., Zagreb, Croatia.
4
Toxicology Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia.
5
Radiation Dosimetry and Radiobiology Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia.
6
Analytical Toxicology and Mineral Metabolism Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia.

Abstract

We studied the toxic effects of glyphosate in vitro on HepG2 cells exposed for 4 and 24 h to low glyphosate concentrations likely to be encountered in occupational and residential exposures [the acceptable daily intake (ADI; 0.5 μg/mL), residential exposure level (REL; 2.91 μg/mL) and occupational exposure level (OEL; 3.5 μg/mL)]. The assessments were performed using biomarkers of oxidative stress, CCK-8 colorimetric assay for cell proliferation, alkaline comet assay and cytokinesis-block micronucleus (CBMN) cytome assay. The results obtained indicated effects on cell proliferation, both at 4 and 24 h. The levels of primary DNA damage after 4-h exposure were lower in treated vs. control samples, but were not significantly changed after 24 h. Using the CBMN assay, we found a significantly higher number of MN and nuclear buds at ADI and REL after 4 h and a lower number of MN after 24 h. The obtained results revealed significant oxidative damage. Four-hour exposure resulted in significant decrease at ADI [lipid peroxidation and glutathione peroxidase (GSH-Px)] and OEL [lipid peroxidation and level of total antioxidant capacity (TAC)], and 24-h exposure in significant decrease at OEL (TAC and GSH-Px). No significant effects were observed for the level of reactive oxygen species (ROS) and glutathione (GSH) for both treatment, and for 24 h for lipid peroxidation. Taken together, the elevated levels of cytogenetic damage found by the CBMN assay and the mechanisms of primary DNA damage should be further clarified, considering that the comet assay results indicate possible cross-linking or DNA adduct formation.

KEYWORDS:

Comet assay; Cytochalasin B-blocked micronucleus assay; Cytotoxicity; Genotoxicity; Glyphosate; HepG2; Low doses; Oxidative damage

PMID:
28667585
DOI:
10.1007/s11356-017-9438-y
[Indexed for MEDLINE]

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