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Mol Phylogenet Evol. 2017 Sep;114:234-248. doi: 10.1016/j.ympev.2017.06.017. Epub 2017 Jun 27.

Phylogenetic analysis of Placobdella (Hirudinea: Rhynchobdellida: Glossiphoniidae) with consideration of COI variation.

Author information

1
Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario M5S 2B4, Canada; Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, Ontario M5S 2C6, Canada. Electronic address: danielle.decarle@mail.utoronto.ca.
2
Laboratorio de Helmintología, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Copilco, Coyoacán, A.P. 70-153, Ciudad de México C.P. 14510, Mexico.
3
Richard Gilder Graduate School, American Museum of Natural History, New York, NY 10024, USA; Sackler Institute for Comparative Genomics, and Division of Invertebrate Zoology, American Museum of Natural History, Central Park West at 79thStreet, New York, NY 10024, USA.
4
Sackler Institute for Comparative Genomics, and Division of Invertebrate Zoology, American Museum of Natural History, Central Park West at 79thStreet, New York, NY 10024, USA.
5
Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario M5S 2B4, Canada; Department of Natural History, Royal Ontario Museum, 100 Queen's Park, Toronto, Ontario M5S 2C6, Canada.

Abstract

Placobdella is a genus of blood-feeding leeches in the family Glossiphoniidae. Historically, species of Placobdella have posed difficulty for systematists owing to a lack of informative morphological characters and the preponderance of inadequate or incomplete species descriptions. Here, we conduct a phylogenetic analysis of 55 individuals representing 20 of the 24 currently recognized nominal taxa using COI, ND1, 12S rDNA and ITS sequences under parsimony, maximum likelihood and Bayesian inference. We also examine the isolated COI phylogeny for the genus using an expanded dataset encompassing three additional species not included in the concatenated dataset. Finally, we assess genetic variation at the COI locus to validate initial specimen identifications and estimate how COI variation may reflect species boundaries. We conclude that Placobdella is a monophyletic group that places as the sister group to a clade formed by the genera Haementeria and Helobdella. We discuss the evolutionary implications of several internal relationships that are robustly resolved by all three optimality criteria, paying particular attention to the apparent fluidity of morphological characters exhibited by members of Placobdella. We also find preliminary evidence for the presence of cryptic and undescribed diversity within the genus.

KEYWORDS:

COI variation; Leech; Phylogeny; Placobdella; Species delimitation

PMID:
28666786
DOI:
10.1016/j.ympev.2017.06.017
[Indexed for MEDLINE]

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