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Science. 2017 Jun 30;356(6345):1379-1383. doi: 10.1126/science.aam5887.

Decoding of position in the developing neural tube from antiparallel morphogen gradients.

Author information

1
Institute of Science and Technology IST Austria, 3400 Klosterneuburg, Austria.
2
Institute of Bioengineering, School of Life Sciences, and School of Engineering, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
3
Institute of Science and Technology IST Austria, 3400 Klosterneuburg, Austria. anna.kicheva@ist.ac.at james.briscoe@crick.ac.uk t.bollenbach@uni-koeln.de.
4
Institute for Theoretical Physics, University of Cologne, Cologne, Germany.
5
Francis Crick Institute, London NW1 1AT, UK. anna.kicheva@ist.ac.at james.briscoe@crick.ac.uk t.bollenbach@uni-koeln.de.
6
Francis Crick Institute, London NW1 1AT, UK.

Abstract

Like many developing tissues, the vertebrate neural tube is patterned by antiparallel morphogen gradients. To understand how these inputs are interpreted, we measured morphogen signaling and target gene expression in mouse embryos and chick ex vivo assays. From these data, we derived and validated a characteristic decoding map that relates morphogen input to the positional identity of neural progenitors. Analysis of the observed responses indicates that the underlying interpretation strategy minimizes patterning errors in response to the joint input of noisy opposing gradients. We reverse-engineered a transcriptional network that provides a mechanistic basis for the observed cell fate decisions and accounts for the precision and dynamics of pattern formation. Together, our data link opposing gradient dynamics in a growing tissue to precise pattern formation.

PMID:
28663499
PMCID:
PMC5568706
DOI:
10.1126/science.aam5887
[Indexed for MEDLINE]
Free PMC Article

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