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J Lipid Res. 2017 Sep;58(9):1822-1833. doi: 10.1194/jlr.M076141. Epub 2017 Jun 29.

Secretory phospholipase A2 group IIA modulates insulin sensitivity and metabolism.

Author information

1
Departments of Pharmacology, College of Medicine, University of Tennessee Health Science Center, Memphis, TN.
2
Department of Veterans Affairs Medical Center, Memphis, TN.
3
Physiology, College of Medicine, University of Tennessee Health Science Center, Memphis, TN.
4
Pediatrics, College of Medicine, University of Tennessee Health Science Center, Memphis, TN.
5
Department of Nutritional Sciences, University of Michigan School of Public Health, Ann Arbor, MI.
6
Department of Infectious Diseases and Immunity, Faculté de Médecine de l'Université Laval, CHUQ Research Center and Division of Rheumatology, Quebec City, Canada.
7
Departments of Pharmacology, College of Medicine, University of Tennessee Health Science Center, Memphis, TN epark@uthsc.edu.

Abstract

Secretory phospholipase A2 group IIA (PLA2G2A) is a member of a family of secretory phospholipases that have been implicated in inflammation, atherogenesis, and antibacterial actions. Here, we evaluated the role of PLA2G2A in the metabolic response to a high fat diet. C57BL/6 (BL/6) mice do not express PLA2g2a due to a frameshift mutation. We fed BL/6 mice expressing the human PLA2G2A gene (IIA+ mice) a fat diet and assessed the physiologic response. After 10 weeks on the high fat diet, the BL/6 mice were obese, but the IIA+ mice did not gain weight or accumulate lipid. The lean mass in chow- and high fat-fed IIA+ mice was constant and similar to the BL/6 mice on a chow diet. Surprisingly, the IIA+ mice had an elevated metabolic rate, which was not due to differences in physical activity. The IIA+ mice were more insulin sensitive and glucose tolerant than the BL/6 mice, even when the IIA+ mice were provided the high fat diet. The IIA+ mice had increased expression of uncoupling protein 1 (UCP1), sirtuin 1 (SIRT1), and PPARγ coactivator 1α (PGC-1α) in brown adipose tissue (BAT), suggesting that PLA2G2A activates mitochondrial uncoupling in BAT. Our data indicate that PLA2G2A has a previously undiscovered impact on insulin sensitivity and metabolism.

KEYWORDS:

hepatic steatosis; high fat diet; insulin resistance; obesity

PMID:
28663239
PMCID:
PMC5580896
DOI:
10.1194/jlr.M076141
[Indexed for MEDLINE]
Free PMC Article

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