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Nat Methods. 2017 Jun 29;14(7):657-661. doi: 10.1038/nmeth.4344.

Assessing phototoxicity in live fluorescence imaging.

Author information

1
School of Biological Sciences, University of Essex, Colchester, UK.
2
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
3
UCD School of Biomolecular and Biomedical Science, University College Dublin, Dublin, Ireland.
4
National Institute of Biomedical Imaging and Bioengineering, US National Institutes of Health, Bethesda, Maryland, USA.

Abstract

Are the answers to biological questions obtained via live fluorescence microscopy substantially affected by phototoxicity? Although a single set of standards for assessing phototoxicity cannot exist owing to the breadth of samples and experimental questions associated with biological imaging, we need quantitative, practical assessments and reporting standards to ensure that imaging has a minimal impact on observed biological processes and sample health. Here we discuss the problem of phototoxicity in biology and suggest guidelines to improve its reporting and assessment.

PMID:
28661494
DOI:
10.1038/nmeth.4344
[Indexed for MEDLINE]

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