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Microbiologyopen. 2017 Aug;6(4). doi: 10.1002/mbo3.491. Epub 2017 Jun 28.

Relative variations of gut microbiota in disordered cholesterol metabolism caused by high-cholesterol diet and host genetics.

Bo T1,2, Shao S2,3, Wu D2, Niu S2,4, Zhao J2,3, Gao L1,2.

Author information

1
Scientific Center, Shandong Provincial Hospital affiliated to Shandong University, Jinan, Shandong, China.
2
Institute of Endocrinology, Shandong Academy of Clinical Medicine, Jinan, Shandong, China.
3
Department of Endocrinology and Metabolism, Shandong Provincial Hospital affiliated to Shandong University, Jinan, Shandong, China.
4
Department of Endocrinology, Lin Yi People's Hospital affiliated to Shandong University, Linyi, Shandong, China.

Abstract

Recent studies performed provide mechanistic insight into effects of the microbiota on cholesterol metabolism, but less focus was given to how cholesterol impacts the gut microbiota. In this study, ApoE-/- Sprague Dawley (SD) rats and their wild-type counterparts (n = 12) were, respectively, allocated for two dietary condition groups (normal chow and high-cholesterol diet). Total 16S rDNA of fecal samples were extracted and sequenced by high-throughput sequencing to determine differences in microbiome composition. Data were collected and performed diversity analysis and phylogenetic analysis. The influence of cholesterol on gut microbiota was discussed by using cholesterol dietary treatment as exogenous cholesterol disorder factor and genetic modification as endogenous metabolic disorder factor. Relative microbial variations were compared to illustrate the causality and correlation of cholesterol and gut microbiota. It turned out comparing to genetically modified rats, exogenous cholesterol intake may play more effective role in changing gut microbiota profile, although the serum cholesterol level of genetically modified rats was even higher. Relative abundance of some representative species showed that the discrepancies due to dietary variation were more obvious, whereas some low abundance species changed because of genetic disorders. Our results partially demonstrated that gut microbiota are relatively more sensitive to dietary variation. Nevertheless, considering the important effect of bacteria in cholesterol metabolism, the influence to gut flora by "genetically caused cholesterol disorder" cannot be overlooked. Manipulation of gut microbiota might be an effective target for preventing cholesterol-related metabolic disorders.

KEYWORDS:

gut microbiota; high-cholesterol diet; metabolism disorder

PMID:
28660729
PMCID:
PMC5552918
DOI:
10.1002/mbo3.491
[Indexed for MEDLINE]
Free PMC Article

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