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J Am Coll Nutr. 2017 Jul;36(5):391-398. doi: 10.1080/07315724.2017.1297744. Epub 2017 Jun 28.

Cooked Chickpea Consumption Inhibits Colon Carcinogenesis in Mice Induced with Azoxymethane and Dextran Sulfate Sodium.

Author information

1
a Escuela Nacional de Ciencias Biológicas , Instituto Politécnico Nacional, Delegación Gustavo A. Madero , Ciudad de México.
2
b Cátedra-CONACYT, Facultad de Medicina y Cirugía , Universidad Autónoma Benito Juárez de Oaxaca , Oaxaca de Juárez , México.
3
c Departamento de Biología Celular , Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional , Mexico , México.

Abstract

SCOPE:

The objective of the study was to evaluate the effect of daily consumption of cooked chickpea (2% and 10%) in ICR male mice in which colon cancer was induced with azoxymethane and dextran sulfate sodium.

METHODS AND RESULTS:

The effect of consumption of 2% or 10% cooked chickpeas on carcinogenesis-induced colon azoxymethane (AOM)/dextran sulfate sodium (DSS) in ICR mice was determined. Protein oxidation and lipids were determined by colorimetric methods and oxidation of DNA through the identification of adducts 8-hydroxy-2'-desoxiguanosine and proliferation markers (proliferating cell nuclear antigen [PCNA], Ki-67, and β-catenin), and inflammation (cyclooxygenase [COX]-2 and inducible nitric oxide synthase [iNOS]) were identified by immunohistochemistry reactions. The results showed the protective effect of daily consumption of rich cooked chickpeas in the carcinogenesis process, decreasing lipid, protein, and DNA oxidation and decreasing the expression of inflammatory enzymes (COX-2 and iNOS) as well as β-catenin, one of the most important oncogenic proteins in colon cancer. Animals that were fed with the 10% chickpea diet showed an inhibition in cellular proliferation (Ki-67 and PCNA expression).

CONCLUSIONS:

The addition of cooked chickpea seed (2% and 10%) to the daily diet is proposed as a chemopreventive agent against colon cancer.

KEYWORDS:

Carcinogenesis; chemopreventive; chickpea; legumes

PMID:
28657468
DOI:
10.1080/07315724.2017.1297744
[Indexed for MEDLINE]

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