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Tumour Biol. 2017 Jun;39(6):1010428317704821. doi: 10.1177/1010428317704821.

Overexpression of WDFY2 inhibits prostate cancer cell growth and migration via inactivation of Akt pathway.

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1 Department of Urology, Huashan Hospital, Fudan University, Shanghai, P.R. China.
2 Institutes of Biomedical Sciences, Fudan University, Shanghai, P.R. China.
3 Department of Pathology, Affiliated Yuhuangding Hospital of Qingdao University, Qingdao, P.R. China.
4 Program for Personalized Cancer Care and Department of Surgery, North Shore University Health System, Evanston, IL, USA.


Prostate cancer is the most commonly diagnosed malignancy and is the second leading deadly reason among male cancer. WDFY2, which is found to be a cancer-specific fusion gene with CDKN2D in ovarian cancer, is a new gene with unknown function in carcinogenesis. In this study, we investigated the role of WDFY2 in prostate cancer development. We examined WDFY2 expression in human prostate tissue specimens and prostate cancer cell lines BPH-1, LNCaP, PC3, and DU-145. Overexpression of WDFY2 was performed to evaluate the role of WDFY2 in cell proliferation, migration, and colony formation of prostate cancer cells. We analyzed the clinical impact and prognosis of WDFY2 expression on the progress of prostate cancer through data from online datasets. Our results showed that WDFY2 had lower expression level in prostate tumors than in normal tissues. Overexpression of WDFY2 in prostate cancer cells DU145 and PC-3 led to the suppression of cancer cell migration and colony formation. Furthermore, we found that WDFY2 exerted its role by suppressing the activity of Akt pathway other than the epithelial-mesenchymal transition progression. In conclusion, we have uncovered WDFY2 as a tumor suppressor gene and a new potential biomarker for cancer progression. Our results showed that WDFY2 inhibited cancer cell colony formation and migration via suppressing Akt pathway, making it a potential new therapeutic target in prostate cancer.


Akt pathway; WDFY2; migration and colony formation; prostate cancer

[Indexed for MEDLINE]

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