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Prostaglandins Leukot Essent Fatty Acids. 2017 Jun;121:76-87. doi: 10.1016/j.plefa.2017.06.007. Epub 2017 Jun 15.

Effect of DHA supplementation on oxylipin levels in plasma and immune cell stimulated blood.

Author information

1
Institute of Food Science and Human Nutrition, Faculty of Natural Sciences, Leibniz University Hannover, Germany. Electronic address: Schuchardt@nutrition.uni-hannover.de.
2
Institute for Food Toxicology and Analytical Chemistry, University of Veterinary Medicine Hannover, Germany.
3
Institute of Food Science and Human Nutrition, Faculty of Natural Sciences, Leibniz University Hannover, Germany.
4
Institute for Food Toxicology and Analytical Chemistry, University of Veterinary Medicine Hannover, Germany; Faculty of Mathematics and Natural Sciences, University of Wuppertal, Germany.

Abstract

INTRODUCTION:

EPA and DHA cause different physiological effects, which are in many cases mediated via their oxidative metabolites (oxylipins). However, metabolism studies investigating the effect of either EPA or DHA on comprehensive oxylipin patterns are lacking.

MATERIAL AND METHODS:

The short and long term (1, 3, 6, and 12 week) effect of 1076mg/d DHA (free of EPA) on free (unesterified) oxylipin concentrations in plasma and lipopolysacharid (LPS) stimulated blood of 12 healthy men (mean age 25.1 ± 1.5 years) was investigated.

RESULTS:

After DHA supplementation, plasma levels of all DHA-oxylipins (HDHAs, EpDPEs, DiHDPEs) significantly increased (up to 600%) in a time-dependent fashion. Oxylipins of EPA and arachidonic acid (AA) were also affected. Whereas a slight increase in several EPA-derived hydroxy-FAs (including the RvE1 precursor 18-HEPE) and dihydroxy-FAs was observed after DHA supplementation, a trend to a slight decline in AA-derived oxylipin levels was found. In LPS stimulated blood, it is shown that DHA supplementation significantly reduces the ability of immune cells to form AA-derived COX (TXB2 and PGB2) and 12-LOX (12-HETE) eicosanoids. While no increase in EPA COX metabolites was found, n-3 PUFA 12-LOX metabolites of EPA (12-HEPE) and DHA (14-HDHA) were highly induced.

CONCLUSION:

We demonstrated that DHA supplementation causes a time-dependent shift in the entire oxylipin profile suggesting a cross-linked metabolism of PUFAs and subsequent formation of oxygenated lipid mediators.

KEYWORDS:

DHA; EPA; Free oxylipins; Immune cell stimulation; LC n-3 PUFA; Lipid mediators

PMID:
28651702
DOI:
10.1016/j.plefa.2017.06.007
[Indexed for MEDLINE]

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