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Bioresour Technol. 2017 Oct;241:1043-1049. doi: 10.1016/j.biortech.2017.06.002. Epub 2017 Jun 6.

An efficient production of high-pure xylooligosaccharides from corncob with affinity adsorption-enzymatic reaction integrated approach.

Author information

1
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China.
2
School of Pharmaceutical Sciences, Nanjing Tech University, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China.
3
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China; Jiangsu National Synergetic Innovation Center for Advanced Materials, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China. Electronic address: wubin1977@njtech.edu.cn.
4
Jiangsu National Synergetic Innovation Center for Advanced Materials, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China; School of Pharmaceutical Sciences, Nanjing Tech University, 30 Puzhunan Road, Nanjing 211816, Jiangsu, China.

Abstract

Xylooligosaccharides (XOS) are high value-added ingredients for functional foods and they have potential use as prebiotics. In order to reduce the production cost of XOS, we constructed a fusion enzyme consisting of expansin and endo-xylanase from B. subtilis Lucky9 to produce high-pure XOS with affinity adsorption-enzymatic reaction integrated approach. By optimization of inserting linker, the specific xylanase activity of fusion enzyme with R2 linker was increased 1.28-fold than that of native xylanase. Electrophoretic pure fusion enzyme was separated from crude enzyme extracts by affinity adsorption on corncob substrate, with 90.3% recovery in optimized conditions. Then, the fusion enzyme-corncob mixture was directly hydrolyzed. The yield of XOS reached 6.91mg/mL. The production of XOS with high purity of 91.7% was obtained by one step of centrifugation. This affinity adsorption-enzymatic reaction integrated XOS production approach provides a green route to alternative XOS traditional enzymatic production.

KEYWORDS:

Adsorption-enzymatic integrated reaction; Fusion enzyme; High purity; Xylooligosaccharides

PMID:
28651320
DOI:
10.1016/j.biortech.2017.06.002
[Indexed for MEDLINE]

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