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J Spinal Cord Med. 2018 Jan;41(1):10-16. doi: 10.1080/10790268.2017.1314880. Epub 2017 Jun 26.

Bone mesenchymal stem cell-conditioned medium decreases the generation of astrocytes during the process of neural stem cells differentiation.

Author information

1
a Department of Spinal Surgery , The First Affiliated Hospital of Anhui Medical University , Hefei , China.
2
b Department of Medical Imaging , Bengbu Medical College , Bengbu , China.
3
c School of Life Science , Anhui Medical University , Heifei , China.

Abstract

OBJECTIVES:

The aim of this study was to investigate the effect of bone mesenchymal stem cell (BMSC) conditioned medium (CM) and Bone morphogenetic protein-4 (BMP-4) on the generation of astrocytes during the process of NSCs differentiation.

DESIGN:

Neural stem cells (NSCs) were grown under different culture conditions.

SETTING:

The First Affiliated Hospital of Anhui Medical University, Hefei, China.

OUTCOME MEASURES:

The study consisted of four groups: NSCs cultured under control conditions (group 1) or with the addition of BMSC-CM (group 2);(BMP-4) (group 3) or both (group 4).The expression of glial fibrillary acidic protein (GFAP) was detected by immunocytochemical staining and Western blotting.

RESULTS:

The expression of GFAP was higher in Group3 and lower in Group 2 compared to that in Group 1. The expression of GFAP in Group 4 was intermediate between that of Group 2 and Group 3.

CONCLUSIONS:

These results suggest that BMSC-CM can decrease the generation of astrocytes and that the inhibition of the (BMP-4) /Smad1/5/8 signaling pathway may be the underlying mechanism. This phenomenon may be mediated by increasing the expression of Smad6.

KEYWORDS:

Astrocytes; BMP-4/SMAD signaling pathway; Bone mesenchymal stem cells conditioned medium; Bone morphogenetic protein-4; Neural stem cell differentiation

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