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Clin Immunol. 2017 Oct;183:24-35. doi: 10.1016/j.clim.2017.06.008. Epub 2017 Jun 23.

Identification of evidence for autoimmune pathology of bilateral sudden sensorineural hearing loss using proteomic analysis.

Author information

1
Department of Otorhinolaryngology, Ilsan Paik Hospital, Inje University College of Medicine, Goyang, Republic of Korea.
2
Research Center for Human Natural Defense System, Yonsei University College of Medicine, Seoul, Republic of Korea.
3
Department of Otorhinolaryngology, Ilsan Paik Hospital, Inje University College of Medicine, Goyang, Republic of Korea; Department of Anatomy, Yonsei University College of Medicine, Seoul, Republic of Korea; BK21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, Republic of Korea.
4
Department of Otorhinolaryngology, Inha University College of Medicine, Incheon, Republic of Korea.
5
Department of Otorhinolaryngology, Ilsan Paik Hospital, Inje University College of Medicine, Goyang, Republic of Korea; The Airway Mucus Institute, Yonsei University College of Medicine, Seoul, Republic of Korea.
6
Department of Otorhinolaryngology, Ilsan Paik Hospital, Inje University College of Medicine, Goyang, Republic of Korea; The Airway Mucus Institute, Yonsei University College of Medicine, Seoul, Republic of Korea. Electronic address: fledermaus@yuhs.ac.

Abstract

Sudden sensorineural hearing loss (S-SNHL) is an inner ear disorder with an abrupt hearing loss occurring <3days. The pathologic mechanism of the disease remains unclear, although autoimmunity has been regarded as one of the suggested causes, especially in bilateral form. In this study, we aimed to provide evidence for the involvement of autoimmunity in bilateral S-SNHL using proteomic approaches such as ProtoArray®, western blotting, immunoprecipitation, and liquid column mass spectrometry for mass screening of candidate antigens and autoantibodies based on the hypothesis that multiple autoantibodies and target antigens must exist in order for autoimmune bilateral S-SNHL to develop. As the final outcome, we have proven the involvement of autoimmunity in the disease, and investigated the existence of circulating autoantibodies and candidate antigens. These findings could provide basic evidence necessary for the development of diagnostic biomarkers as well as the understanding of the pathological mechanisms underlying bilateral S-SNHL. S-SNHL: sudden sensorineural hearing loss; LC-MS: liquid chromatography-mass spectrometry; MS: mass spectrometry; autoAb: autoantibody; 1-DE: one-dimensional electrophoresis.

KEYWORDS:

Autoantibody; Autoimmune; Bilateral sudden sensorineural hearing loss; Inner ear

PMID:
28648633
DOI:
10.1016/j.clim.2017.06.008
[Indexed for MEDLINE]

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