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BMC Immunol. 2017 Jun 24;18(1):33. doi: 10.1186/s12865-017-0215-y.

The imbalance of Th17/Treg cells is involved in the progression of nonalcoholic fatty liver disease in mice.

Author information

1
Laboratory of Digestive Disease, the First Affiliated Hospital of Zhejiang Chinese Medical University, 54,Youdian Road, Hangzhou, 310006, Zhejiang Province, People's Republic of China.
2
Department of Equipment, the Third People's Hospital of Hangzhou, 38,Westlake Road, Hangzhou, 310009, People's Republic of China.
3
The University of Melbourne, Melbourne, VIC, 3000, Australia.
4
Department of Preparation Center, the First Affiliated Hospital of Zhejiang Chinese Medical University, 54,Youdian Road, Hangzhou, 310006, Zhejiang Province, People's Republic of China.
5
Department of Gastroenterology, the Second People's Hospital of Yuhang District of Hangzhou, 80,Anle Road, Yuhang District of Hangzhou, 311100, People's Republic of China.
6
Laboratory of Digestive Disease, the First Affiliated Hospital of Zhejiang Chinese Medical University, 54,Youdian Road, Hangzhou, 310006, Zhejiang Province, People's Republic of China. zhiyunchen63@163.com.
7
Department of Laboratory Medicine, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, Zhejiang, People's Republic of China.

Abstract

BACKGROUND:

Non-alcoholic fatty liver disease (NAFLD) is a common, chronic liver disease worldwide. Recent studies have shown that T helper (Th) 17 and regulatory T (Treg) cells play critical roles in various disorders of liver inflammation. Here, we explored the value of polyene phosphatidylcholine capsules (PPC) for regulating the imbalance of Th17/Treg cells in the pathogenesis of mice with NAFLD.

METHODS:

C57BL/6 mice were randomly divided into three groups as follows:normal diet (ND), high-fat diet (HF),and HF plus PPC(HF + PPC). The frequencies of splenic Th17 and Treg cells were measured by flow cytometry, and their related cytokines were analyzed by CBA and real-time PCR.

RESULTS:

At the end of 24 weeks, mice in the HF group had a higher frequency of intrahepatic Th17 cells,and a lower proportion of Treg cells compared with the ND group. The levels of Th17 cell-related cytokines (IL-6, IL-17 and IL-23) in serum and in liver tisse were increased,and the hepatic mRNA levels of RORγt, STAT3 and IL-6 were also increased. By contrast,the FoxP3 mRNA level was decreased in the HF group. Moreover, significant pathological and biochemical changes in the liver, as well as serum biochemical changes, were found in mice with NAFLD. Interestingly, following treatment with PPC, the levels of liver inflammation,frequencies of Th17/Treg cells and associated cytokines,and biochemical data were significantly altered.

CONCLUSION:

These findings demonstrate a critical role for PPC in partially attenuating liver inflammatory responses in mice with NAFLD that involves the imbalance of Treg/Th17 cells and associated cytokines.

KEYWORDS:

Nonalcoholic fatty liver disease; Th17 cells; Treg cells

PMID:
28646856
PMCID:
PMC5483270
DOI:
10.1186/s12865-017-0215-y
[Indexed for MEDLINE]
Free PMC Article

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