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Curr Opin Microbiol. 2017 Jun;37:110-119. doi: 10.1016/j.mib.2017.05.010. Epub 2017 Jun 21.

Conformational regulation of CRISPR-associated nucleases.

Author information

1
Department of Chemistry and Biochemistry, Utah State University, Logan, UT 84322, United States. Electronic address: ryan.jackson@usu.edu.
2
Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, United States. Electronic address: pbgvanerp@gmail.com.
3
Caribou Biosciences, Inc., Berkeley, CA 94710, United States.
4
Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, United States. Electronic address: bwiedenheft@gmail.com.

Abstract

Adaptive immune systems in bacteria and archaea rely on small CRISPR-derived RNAs (crRNAs) to guide specialized nucleases to foreign nucleic acids. The activation of these nucleases is controlled by a series of molecular checkpoints that ensure precise cleavage of nucleic acid targets, while minimizing toxic off-target cleavage events. In this review, we highlight recent advances in understanding regulatory mechanisms responsible for controlling the activation of these nucleases and identify emerging regulatory themes conserved across diverse CRISPR systems.

PMID:
28646675
PMCID:
PMC5687066
DOI:
10.1016/j.mib.2017.05.010
[Indexed for MEDLINE]
Free PMC Article

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