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Methods Mol Biol. 2017;1630:91-100. doi: 10.1007/978-1-4939-7128-2_8.

Generation of Knock-in Mouse by Genome Editing.

Author information

1
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. awtrfj@mail.ecc.u-tokyo.ac.jp.

Abstract

Knock-in mice are useful for evaluating endogenous gene expressions and functions in vivo. Instead of the conventional gene-targeting method using embryonic stem cells, an exogenous DNA sequence can be inserted into the target locus in the zygote using genome editing technology. In this chapter, I describe the generation of epitope-tagged mice using engineered endonuclease and single-stranded oligodeoxynucleotide through the mouse zygote as an example of how to generate a knock-in mouse by genome editing.

KEYWORDS:

Engineered endonuclease; Genome editing; Knock-in mice; Single-stranded oligodeoxynucleotide (ssODN); Zygote

PMID:
28643252
DOI:
10.1007/978-1-4939-7128-2_8
[Indexed for MEDLINE]

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