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G3 (Bethesda). 2017 Aug 7;7(8):2807-2820. doi: 10.1534/g3.117.044099.

Phenotypic Diagnosis of Lineage and Differentiation During Sake Yeast Breeding.

Author information

1
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, 277-8562, Japan.
2
Department of Genetics, Genomics and Microbiology, University of Strasbourg, Centre National de la Recherche Scientifique, 67083, France.
3
Brewing Microbiology Division, National Research Institute of Brewing, Higashi-Hiroshima, 739-0046, Japan.
4
Research Institute, Ozeki Corporation Ltd., Nishinomiya, 663-8227, Japan.
5
Brewing Society of Japan, Kita-ku, 114-0023, Japan.
6
Research and Development Department, Asahi Sake Brewing Co., Ltd., Nagaoka, 949-5494, Japan.
7
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, 739-8530, Japan.
8
Department of Biological Chemistry and Food Sciences, Iwate University, Morioka, 020-8550, Japan.
9
Pharmaceutical Medical Business Sciences, Nihon Pharmaceutical University, Bunkyo-ku, 113-0034, Japan.
10
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0192, Japan.
11
Brewing Microbiology Division, National Research Institute of Brewing, Higashi-Hiroshima, 739-0046, Japan akao_t@nrib.go.jp ohya@k.u-tokyo.ac.jp.
12
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, 277-8562, Japan akao_t@nrib.go.jp ohya@k.u-tokyo.ac.jp.

Abstract

Sake yeast was developed exclusively in Japan. Its diversification during breeding remains largely uncharacterized. To evaluate the breeding processes of the sake lineage, we thoroughly investigated the phenotypes and differentiation of 27 sake yeast strains using high-dimensional, single-cell, morphological phenotyping. Although the genetic diversity of the sake yeast lineage is relatively low, its morphological diversity has expanded substantially compared to that of the Saccharomycescerevisiae species as a whole. Evaluation of the different types of breeding processes showed that the generation of hybrids (crossbreeding) has more profound effects on cell morphology than the isolation of mutants (mutation breeding). Analysis of phenotypic robustness revealed that some sake yeast strains are more morphologically heterogeneous, possibly due to impairment of cellular network hubs. This study provides a new perspective for studying yeast breeding genetics and micro-organism breeding strategies.

KEYWORDS:

cell morphology; phenotypic diversity; sake yeast; single-cell phenotyping

PMID:
28642365
PMCID:
PMC5555484
DOI:
10.1534/g3.117.044099
[Indexed for MEDLINE]
Free PMC Article

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