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FEBS Lett. 2017 Aug;591(15):2251-2260. doi: 10.1002/1873-3468.12721. Epub 2017 Jul 12.

Subconductance states of mitochondrial chloride channels: implication for functionally-coupled tetramers.

Author information

1
MT Research, Bratislava, Slovak.
2
Institute of Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovak.
3
Institute of Molecular Physiology and Genetics, Centre of Biosciences, Slovak Academy of Sciences, Bratislava, Slovak.

Abstract

Recently, it has been discovered that isoforms of intracellular chloride channels (CLIC) are present in cardiac mitochondria. By reconstituting rat cardiac mitochondrial chloride channels into bilayer lipid membranes, we detected three equally separated subconductance states with conductance increment of 45 pS and < 2% occupancy. The observed rare events of channel decomposition into substates, accompanied by disrupted gating, provide an insight into channel quaternary structure. Our findings suggest that the observed channels work as four functionally coupled subunits with synchronized gating. We discuss the putative connection of channel activity from native mitochondria with the recombinant CLIC channels. However, conclusive evidence is needed to prove this connection.

KEYWORDS:

chloride channel; mean-variance analysis; mitochondria

PMID:
28640976
DOI:
10.1002/1873-3468.12721
[Indexed for MEDLINE]
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