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An Acad Bras Cienc. 2017 Apr-Jun;89(2):927-938. doi: 10.1590/0001-3765201720160411.

Psychotria viridis: Chemical constituents from leaves and biological properties.

Author information

1
Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
2
Setor Técnico-Científico do Departamento de Polícia Federal de Minas Gerais, Rua Nascimento Gurgel, 30, 30441-170 Belo Horizonte, MG, Brazil.
3
Universidade do Estado de Minas Gerais, Unidade de Divinópolis, Avenida Paraná, 3001, 35501-170 Divinópolis, MG, Brazil.
4
Departamento de Farmacologia, ICB, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil.
5
Departamento de Farmácia, Escola de Farmácia, Universidade Federal de Ouro Preto, Campus Universitário, 35400-000 Ouro Preto, Minas Gerais, Brazil.

Abstract

The phytochemical study of hexane, chloroform and methanol extracts from leaves of Psychotria viridis resulted in the identification of: the pentacyclic triterpenes, ursolic and oleanolic acid; the steroids, 24-methylene-cycloartanol, stigmasterol and β-sitosterol; the glycosylated steroids 3-O-β-D-glucosyl-β-sitosterol and 3-O-β-D-glucosyl-stigmasterol; a polyunsaturated triterpene, squalene; the esters of glycerol, 1-palmitoylglycerol and triacylglycerol; a mixture of long chain hydrocarbons; the aldehyde nonacosanal; the long chain fat acids hentriacontanoic, hexadecanoic and heptadenoic acid; the ester methyl heptadecanoate; the 4-methyl-epi-quinate and two indole alkaloids, N,N-dimethyltryptamine (DMT) and N-methyltryptamine. The chemical structures were determined by means of spectroscopic (IR, 1H and 13C NMR, HSQC, HMBC and NOESY) and spectrometric (CG-MS and LCMS-ESI-ITTOF) methods. The study of biologic properties of P. viridis consisted in the evaluation of the acetylcholinesterase inhibition and cytotoxic activities. The hexane, chloroform, ethyl acetate and methanol extracts, the substances 24-methylene-cycloartanol, DMT and a mixture of 3-O-β-D-glucosyl-β-sitosterol and 3-O-β-D-glucosyl-stigmasterol showed cholinesterase inhibiting activity. This activity induced by chloroform and ethyl acetate extracts was higher than 90%. The methanol and ethyl acetate extracts inhibit the growth and/or induce the death of the tumor cells strains B16F10 and 4T1, without damaging the integrity of the normal cells BHK and CHO. DMT also demonstrated a marked activity against tumor cell strains B16F10 and 4T1.

PMID:
28640347
DOI:
10.1590/0001-3765201720160411
[Indexed for MEDLINE]
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