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Nanomedicine (Lond). 2017 Jul;12(14):1675-1687. doi: 10.2217/nnm-2017-0079. Epub 2017 Jun 21.

Multimodal imaging Gd-nanoparticles functionalized with Pittsburgh compound B or a nanobody for amyloid plaques targeting.

Author information

1
Laboratoire de Chimie et Biologie des Métaux, Université Grenoble Alpes, CEA Life Sciences Division, CNRS, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France.
2
Nano-H S.A.S, 38070 Saint Quentin Fallavier, France.
3
Institut Lumière Matière, University of Lyon, University of Claude Bernard Lyon 1, CNRS, F-69622, Lyon, France.
4
School of Pharmaceutical Sciences, University of Geneva, University of Lausanne Pharmaceutical technology, Quai Ernest-Ansermet 30, 1211 Geneva, Switzerland.
5
Laboratory of Enzymology & Protein Folding, Centre for Protein Engineering, InBioS, University of Liege Sart Tilman, 4000 Liege, Belgium.
6
University of Lyon, University of Claude Bernard Lyon 1, ISPB Faculté de Pharmacie, MATEIS UMR CNRS 5510, 69373 Lyon, France.
7
Centre de Biophysique Moléculaire, CNRS, UPR 4301, Université d'Orléans, Rue Charles Sadron, 45071 Orléans, France.
8
Instituto de Inovação e Investigação em Saúde (I3S), University of Porto, Portugal; Molecular Neurobiology Group, IBMC - Institute for Molecular & Cell Biology, University of Porto, 4150-180 Porto, Portugal.

Abstract

AIM:

Gadolinium-based nanoparticles were functionalized with either the Pittsburgh compound B or a nanobody (B10AP) in order to create multimodal tools for an early diagnosis of amyloidoses.

MATERIALS & METHODS:

The ability of the functionalized nanoparticles to target amyloid fibrils made of β-amyloid peptide, amylin or Val30Met-mutated transthyretin formed in vitro or from pathological tissues was investigated by a range of spectroscopic and biophysics techniques including fluorescence microscopy.

RESULTS:

Nanoparticles functionalized by both probes efficiently interacted with the three types of amyloid fibrils, with KD values in 10 micromolar and 10 nanomolar range for, respectively, Pittsburgh compound B and B10AP nanoparticles. Moreover, they allowed the detection of amyloid deposits on pathological tissues.

CONCLUSION:

Such functionalized nanoparticles could represent promising flexible and multimodal imaging tools for the early diagnostic of amyloid diseases, in other words, Alzheimer's disease, Type 2 diabetes mellitus and the familial amyloidotic polyneuropathy.

KEYWORDS:

PIB; amyloid imaging; amyloidoses; gadolinium-based nanoparticles; nanobody

PMID:
28635419
DOI:
10.2217/nnm-2017-0079
[Indexed for MEDLINE]
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