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Methods Mol Biol. 2017;1612:167-175. doi: 10.1007/978-1-4939-7021-6_12.

3D Hanging Drop Culture to Establish Prostate Cancer Organoids.

Eder T1,2,3, Eder IE4.

Author information

1
Division of Experimental Urology, Department of Urology, Medical University of Innsbruck, Anichstrasse 35, A-6020, Innsbruck, Austria.
2
Translational Radiooncology Laboratory, Department of Radiooncology and Radiotherapy, Charité University Hospital, Berlin, Germany.
3
German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ) Heidelberg, Partner Site Berlin, Berlin, Germany.
4
Division of Experimental Urology, Department of Urology, Medical University of Innsbruck, Anichstrasse 35, A-6020, Innsbruck, Austria. iris.eder@i-med.ac.at.

Abstract

Three-dimensional (3D) cell culture enables the growth of cells in a multidimensional and multicellular manner compared to conventional cell culture techniques. Especially in prostate cancer research there is a big need for more tissue-recapitulating models to get a better understanding of the mechanisms driving prostate cancer as well as to screen for more efficient drugs that can be used for treatment. In this chapter we describe a 3D hanging drop system that can be used to culture prostate cancer organoids as tumor epithelial monocultures and as epithelial-stromal cocultures.

KEYWORDS:

3D culture; Cell viability assay; Drug screening; Epithelial–stromal coculture; Flow cytometry; Hanging drops; Immunohistochemistry; Organoids; Prostate cancer

PMID:
28634942
DOI:
10.1007/978-1-4939-7021-6_12
[Indexed for MEDLINE]

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