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Cell Stress Chaperones. 2017 Nov;22(6):787-797. doi: 10.1007/s12192-017-0809-6. Epub 2017 Jun 20.

Identification of the acclimation genes in transcriptomic responses to heat stress of White Pekin duck.

Author information

1
Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Wanju, 55365, Republic of Korea.
2
Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju, 52828, Republic of Korea.
3
Poultry Research Institute, National Institute of Animal Science, RDA, Pyeongchang, 25342, Republic of Korea.
4
Department of Animal Science, Gyeongsang National University, Jinju, 52828, Republic of Korea.
5
Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA, Wanju, 55365, Republic of Korea. jepark0105@korea.kr.

Abstract

White Pekin duck is an important meat resource in the livestock industries. However, the temperature increase due to global warming has become a serious environmental factor in duck production, because of hyperthermia. Therefore, identifying the gene regulations and understanding the molecular mechanism for adaptation to the warmer environment will provide insightful information on the acclimation system of ducks. This study examined transcriptomic responses to heat stress treatments (3 and 6 h at 35 °C) and control (C, 25 °C) using RNA-sequencing analysis of genes from the breast muscle tissue. Based on three distinct differentially expressed gene (DEG) sets (3H/C, 6H/C, and 6H/3H), the expression patterns of significant DEGs (absolute log2 > 1.0 and false discovery rate < 0.05) were clustered into three responsive gene groups divided into upregulated and downregulated genes. Next, we analyzed the clusters that showed relatively higher expression levels in 3H/C and lower levels in 6H/C with much lower or opposite levels in 6H/3H; we referred to these clusters as the adaptable responsive gene group. These genes were significantly enriched in the ErbB signaling pathway, neuroactive ligand-receptor interaction and type II diabetes mellitus in the KEGG pathways (P < 0.01). From the functional enrichment analysis and significantly regulated genes observed in the enriched pathways, we think that the adaptable responsive genes are responsible for the acclimation mechanism of ducks and suggest that the regulation of phosphoinositide 3-kinase genes including PIK3R6, PIK3R5, and PIK3C2B has an important relationship with the mechanisms of adaptation to heat stress in ducks.

KEYWORDS:

Acclimation; Heat stress; Peking duck; Transcriptome

PMID:
28634817
PMCID:
PMC5655367
DOI:
10.1007/s12192-017-0809-6
[Indexed for MEDLINE]
Free PMC Article

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