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Sci Rep. 2017 Jun 20;7(1):3876. doi: 10.1038/s41598-017-03614-y.

Topical Fibronectin Improves Wound Healing of Irradiated Skin.

Author information

1
Division of Plastic and Reconstructive Surgery, Keck School of Medicine of USC, 1510 San Pablo St., Suite 415, Los Angeles, CA, 90033, USA.
2
Department of Surgery, Keck School of Medicine of USC, 1510 San Pablo St., Suite 415, Los Angeles, CA, 90033, USA.
3
Department of Dermatology, Keck School of Medicine of USC, 1510 San Pablo St., Suite 415, Los Angeles, CA, 90033, USA.
4
Department of Biochemistry and Molecular Biology, Keck School of Medicine of USC, 1510 San Pablo St., Suite 415, Los Angeles, CA, 90033, USA.
5
Norris Comprehensive Cancer Center, Keck School of Medicine of USC, 1510 San Pablo St., Suite 415, Los Angeles, CA, 90033, USA.
6
Center for Craniofacial Molecular Biology, Ostrow School of Dentistry, University of Southern California, 2250 Alcazar St., Los Angeles, 90089, CA, USA.
7
Department of Translational Pathology, MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX, 77030, USA.
8
Division of Plastic and Reconstructive Surgery, Keck School of Medicine of USC, 1510 San Pablo St., Suite 415, Los Angeles, CA, 90033, USA. Alex.Wong@med.usc.edu.
9
Department of Surgery, Keck School of Medicine of USC, 1510 San Pablo St., Suite 415, Los Angeles, CA, 90033, USA. Alex.Wong@med.usc.edu.

Abstract

Wound healing is significantly delayed in irradiated skin. To better understand global changes in protein expression after radiation, we utilized a reverse phase protein array (RPPA) to identify significant changes in paired samples of normal and irradiated human skin. Of the 210 proteins studied, fibronectin was the most significantly and consistently downregulated in radiation-damaged skin. Using a murine model, we confirmed that radiation leads to decreased fibronectin expression in the skin as well as delayed wound healing. Topically applied fibronectin was found to significantly improve wound healing in irradiated skin and was associated with decreased inflammatory infiltrate and increased angiogenesis. Fibronectin treatment may be a useful adjunctive modality in the treatment of non-healing radiation wounds.

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