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Mol Ther. 2017 Aug 2;25(8):1736-1738. doi: 10.1016/j.ymthe.2017.05.021. Epub 2017 Jun 17.

Targeted Deletion of an Entire Chromosome Using CRISPR/Cas9.

Author information

1
School of Biological Science, The University of Adelaide, Adelaide, SA 5005, Australia; Centre for Biomedical Research, Faculty of Medicine, Diponegoro University, Semarang 50271, Indonesia.
2
School of Biological Science, The University of Adelaide, Adelaide, SA 5005, Australia.
3
School of Biological Science, The University of Adelaide, Adelaide, SA 5005, Australia; Robinson Research Institute, The University of Adelaide, Adelaide, SA 5005, Australia.
4
School of Biological Science, The University of Adelaide, Adelaide, SA 5005, Australia; Robinson Research Institute, The University of Adelaide, Adelaide, SA 5005, Australia; South Australian Health and Medical Research Institute, Adelaide, SA 5000, Australia. Electronic address: paul.thomas@adelaide.edu.au.

Abstract

CRISPR/Cas9 genome editing can facilitate efficient deletion of genomic region, but it has not been used to delete an entire chromosome. Here, Adikusuma et al. show proof-of-concept for efficient CRISPR-mediated selective chromosome deletion by removing the centromere or shredding the chromosome arm in mouse embryonic stem cells and zygotes.

PMID:
28633863
PMCID:
PMC5542798
DOI:
10.1016/j.ymthe.2017.05.021
[Indexed for MEDLINE]
Free PMC Article

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