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J Infect Dis. 1985 Sep;152(3):560-5.

Detection of an adherence factor of enteropathogenic Escherichia coli with a DNA probe.

Abstract

A DNA probe to detect genes conferring localized adherence of enteropathogenic Escherichia coli (EPEC) to Hep-2 cells was evaluated by using E. coli isolates from the stools of Peruvian infants with and without diarrhea. The probe was both sensitive and specific and revealed that Hep-2 adherence (because of the EPEC adherence factor [EAF] was more frequent in some O serogroups of EPEC than in others. Those serogroups in which EAF is almost always found have been designated class I EPEC; serogroups in which EAF is rarely found have been designated class II. Both class I (EAF-positive) and class II EPEC are associated with diarrheal disease.

PMID:
2863319
DOI:
10.1093/infdis/152.3.560
[Indexed for MEDLINE]

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